Web Service is the key technical foundation of Web-based distributed computing and application at present. Universal description, discovery and integration(UDDI) is the key technical standard of Web service, which provides the standard of Web service’s register and discovery and solves the problem of Web service’s description, publication and discovery. UDDI specification only offers standard interface and data structure, has no clear definition and implementation mechanism on the security and management of UDDI registry. On the basis of Web service and UDDI technical analysis, UDDI-based application service center (ASC) was designed and implemented.ASC consisted of ASC client, ASC server and ASC administration console, which supported Web service’s publication, discovery and management operation. According to practical requirement, publisher assertion relation match arithmetic was designed and implemented, UDDI’s application program interface was extended, security and management function was provided, therefore a application service registry system was built which had stronger security and apt management.