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可吸收胶原膜的体内免疫反应评价

张林 孙磊 徐梦浛 牛旭锋

张林, 孙磊, 徐梦浛, 等 . 可吸收胶原膜的体内免疫反应评价[J]. 北京航空航天大学学报, 2018, 44(4): 879-886. doi: 10.13700/j.bh.1001-5965.2017.0230
引用本文: 张林, 孙磊, 徐梦浛, 等 . 可吸收胶原膜的体内免疫反应评价[J]. 北京航空航天大学学报, 2018, 44(4): 879-886. doi: 10.13700/j.bh.1001-5965.2017.0230
ZHANG Lin, SUN Lei, XU Menghan, et al. Immunological response evaluation of absorbable collagen membrane in vivo[J]. Journal of Beijing University of Aeronautics and Astronautics, 2018, 44(4): 879-886. doi: 10.13700/j.bh.1001-5965.2017.0230(in Chinese)
Citation: ZHANG Lin, SUN Lei, XU Menghan, et al. Immunological response evaluation of absorbable collagen membrane in vivo[J]. Journal of Beijing University of Aeronautics and Astronautics, 2018, 44(4): 879-886. doi: 10.13700/j.bh.1001-5965.2017.0230(in Chinese)

可吸收胶原膜的体内免疫反应评价

doi: 10.13700/j.bh.1001-5965.2017.0230
基金项目: 

国家自然科学基金 31470915

中央高校基本科研业务费专项资金 YWF-17-BJ-Y-45

详细信息
    作者简介:

    张林  女, 硕士研究生。主要研究方向:生物医用材料

    牛旭锋  男, 博士, 副教授, 博士生导师。主要研究方向:生物医用材料

    通讯作者:

    牛旭锋, E-mail: nxf@buaa.edu.cn

  • 中图分类号: R318.06

Immunological response evaluation of absorbable collagen membrane in vivo

Funds: 

National Natural Science Foundation of China 31470915

the Fundamental Research Funds for the Central Universities YWF-17-BJ-Y-45

More Information
  • 摘要:

    免疫应答反应一直是限制可植入生物材料应用的关键因素之一。实验评估了2种用于骨修复的胶原膜在体内的免疫反应,希望为其临床试验提供依据。在将2种膜皮下植入到BALB/c小鼠后的第14天,与阴性对照(NC,未植入材料)相比,脾和淋巴结没有明显肿大,淋巴结细胞数几乎没差异,而脾细胞数大约是NC的2倍。流式细胞术分析显示植入胶原膜1导致脾中T细胞比例减少了约13%,但是没有影响T细胞亚群,而植入胶原膜2对小鼠的脾细胞组成没有明显影响;2种胶原膜都激活了一定的B细胞,激活率大约为NC小鼠的2倍。淋巴细胞体外增殖实验显示与NC组无显著差异。酶联免疫吸附测试表明胶原膜1导致了第14天血清中的抗体浓度升高至NC小鼠的2倍。局部H&E染色显示2种材料都引起了轻微的细胞浸润。这2种胶原膜引起的免疫反应很微弱,可以被应用于临床试验。

     

  • 图 1  脾和淋巴结形态

    Figure 1.  Images of spleen and lymph nodes

    图 2  脾和淋巴结的免疫细胞计数结果(***p < 0.005)

    Figure 2.  Immune cell population of spleen and lymph nodes (***p < 0.005)

    图 3  流式细胞术分析脾淋巴细胞表型

    Figure 3.  Splenic cells' phenotype analysis by flow cytometry

    图 4  CD4+T细胞、CD8+T细胞和CD19+细胞的Ki67表达情况

    Figure 4.  Ki67 expression of CD4+T lymphocytes, CD8+T lymphocytes and CD19+ lymphocytes

    图 5  脾和淋巴结细胞体外增殖情况(**p < 0.01)

    Figure 5.  Proliferation in vitro of splenic cells and lymph nodes cells (**p < 0.01)

    图 6  血清中IgG浓度随时间的变化

    Figure 6.  Variation of concentration of IgG in serum with time

    图 7  植入部位H&E染色

    Figure 7.  H&E staining of implantation sites

  • [1] TSESIS I, ROSEN E, TAMSE A, et al.Effect of guided tissue regeneration on the outcome of surgical endodontic treatment:A systematic review and meta-analysis[J].Journal of Endodontics, 2011, 37(8):1039-1045. doi: 10.1016/j.joen.2011.05.016
    [2] LEKOVIC V, CAMARGO P M, WEINLAENDER M, et al.Effectiveness of a combination of platelet-rich plasma, bovine porous bone mineral and guided tissue regeneration in the treatment of mandibular grade Ⅱmolar furcations in humans[J].Journal of Clinical Periodontology, 2003, 30(8):746-751. doi: 10.1034/j.1600-051X.2003.00368.x
    [3] SUMITA Y, HONDA M J, OHARA T, et al.Performance of collagen sponge as a 3-D scaffold for tooth-tissue engineering[J].Biomaterials, 2006, 27(17):3238-3248. doi: 10.1016/j.biomaterials.2006.01.055
    [4] LU H K, LEE S Y, LIN F P.Elastic modulus, permeation time and swelling ratio of a new porcine dermal collagen membrane[J].Journal of Periodontal Research, 1998, 33(5):243-248. doi: 10.1111/jre.1998.33.issue-5
    [5] PITARU S, TAL H, SOLDINGER M, et al.Collagen membranes prevent apical migration of epithelium and support new connective tissue attachment during periodontal wound healing in dogs[J].Journal of Periodontal Research, 1989, 24(4):247-253. doi: 10.1111/jre.1989.24.issue-4
    [6] SCULEAN A, NIKOLIDAKIS D, NIKOU G, et al.Biomaterials for promoting periodontal regeneration in human intrabony defects:A systematic review[J].Periodontol 2000, 2015, 68(1):182-216. doi: 10.1111/prd.12086
    [7] LYNN A K, YANNAS I V, BONFIELD W.Antigenicity and immunogenicity of collagen[J].Journal of Biomedical Materials Research Part B:Applied Biomaterials, 2004, 71(2):343-354. http://cn.bing.com/academic/profile?id=3c5c05b343ca6a48f57d249c6cbcec36&encoded=0&v=paper_preview&mkt=zh-cn
    [8] PENG Y Y, GLATTAUER V, RAMSHAW J A, et al.Evaluation of the immunogenicity and cell compatibility of avian collagen for biomedical applications[J].Journal of Biomedical Materials Research Part A, 2010, 93(4):1235-1244. http://cn.bing.com/academic/profile?id=7e3e63aa025ac62f57c0e926a0081b7c&encoded=0&v=paper_preview&mkt=zh-cn
    [9] ZIV O, AVTALION R R, MARGEL S.Immunogenicity of bioactive magnetic nanoparticles:Natural and acquired antibodies[J].Journal of Biomedical Materials Research Part A, 2008, 85(4):1011-1021. http://cn.bing.com/academic/profile?id=1cf9fa32aa65a39f52d13651f12eace0&encoded=0&v=paper_preview&mkt=zh-cn
    [10] DILGIMEN A S, MUSTAFAEVA Z, DEMCHENKO M, et al.Water-soluble covalent conjugates of bovine serum albumin with anionic poly(n-isopropyl-acrylamide) and their immunogenicity[J].Biomaterials, 2001, 22(17):2383-2392. doi: 10.1016/S0142-9612(00)00425-7
    [11] KREISER S, ECKHARDT J, KUHNT C, et al.Murine cd83-positive T cells mediate suppressor functions in vitro and in vivo[J].Immunobiology, 2015, 220(2):270-279. doi: 10.1016/j.imbio.2014.08.005
    [12] O'DONNELL H, PHAM O H, LI L X, et al.Toll-like receptor and inflammasome signals converge to amplify the innate bactericidal capacity of t helper 1 cells[J].Immunity, 2014, 40(2):213-224. doi: 10.1016/j.immuni.2013.12.013
    [13] WELCH R J, LITWIN C M.A comparison of brucella igg and igm elisa assays with agglutination methodology[J].Journal of Clinical Laboratory Analysis, 2010, 24(3):160-162. doi: 10.1002/jcla.v24:3
    [14] LIU H, WISE S G, RNJAK-KOVACINA J, et al.Biocompatibility of silk-tropoelastin protein polymers[J].Biomaterials, 2014, 35(19):5138-5147. doi: 10.1016/j.biomaterials.2014.03.024
    [15] LIU L, KUFFOVA L, GRIFFITH M, et al.Immunological responses in mice to full-thickness corneal grafts engineered from porcine collagen[J].Biomaterials, 2007, 28(26):3807-3814. doi: 10.1016/j.biomaterials.2007.04.025
    [16] PATINO M G, NEIDERS M E, ANDREANA S, et al.Cellular inflammatory response to porcine collagen membranes[J].Journal of Periodontal Research, 2003, 38(5):458-464. doi: 10.1034/j.1600-0765.2003.00017.x
    [17] 方哲翔, 王建华, 袁平, 等.医用胶原修复膜病毒灭活/去除工艺的验证和评价[J].中国生物制品学杂志, 2016, 29(12):1341-1345. http://www.doc88.com/p-9354744381199.html

    FANG Z X, WANG J H, YUAN P, et al.Validation and evaluation of inactivation/removal of medical collagen repair membrane[J].Chinese Journal of Biologicals, 2016, 29(12):1341-1345(in Chinese). http://www.doc88.com/p-9354744381199.html
    [18] 王朋.过氧化氢消毒灭菌技术介绍[J].化工与医药工程, 2012, 33(6):51-53. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=yygcsj201206012

    WANG P.Introduction of sterilization technology with vaporized hydroge peroxide[J].Chemical and Pharmaceutical Engineering, 2012, 33(6):51-53(in Chinese). http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=yygcsj201206012
    [19] 龚居霞, 刘新华, 但卫华, 等.过氧化氢处理对Ⅰ型胶原结构和性能的影响[J].中国皮革, 2015(1):10-13. http://www.cnki.com.cn/Article/CJFDTotal-ZGPG201501005.htm

    GONG J X, LIU X H, DAN W H, et al.Effects of hydrogen peroxide treatment on structure and performance of type Ⅰ collagen[J].China Leather, 2015(1):10-13(in Chinese). http://www.cnki.com.cn/Article/CJFDTotal-ZGPG201501005.htm
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出版历程
  • 收稿日期:  2017-04-13
  • 录用日期:  2017-05-19
  • 网络出版日期:  2018-04-20

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