A method of simulation and flight test for seeker-s particular trajectory in terminal guidance was presented. In the flight simulation of trajectory in terminal guidance, height decrease by the entry maneuver need to be achievably small, and precision request of tracking in the diving flight phase should be satisfied. A set of nonlinear 6-DOF equations was used, in which the coupling between longitude and lateral motion was considered. To keep precise tracking in the diving and flight security with large magnitude of flight speed, dive angle, height altering, 3 types of stick roll control were analyzed, and control scheme satisfies request of precision was given. In the entry phase, bank angle was firstly changed to 180°. After satisfying dive angle of the trajectory by elevator control, bank angle returned to 0°. Flight test project was accomplished to verify the reliability and availability of the control scheme. Results of flight simulation and test show that the method presented could improve flight test efficiency and the precision of aircraft tracking particular trajectory with guaranteed flight security.