北京航空航天大学学报 ›› 2012, Vol. ›› Issue (9): 1186-1188,1193.

• 论文 • 上一篇    下一篇

基于数字全息的活体细胞动态相衬显微观察

刘烁, 潘锋, 肖文   

  1. 北京航空航天大学 仪器科学与光电工程学院, 北京 100191
  • 收稿日期:2011-09-20 出版日期:2012-09-30 发布日期:2012-09-27
  • 基金资助:
    国家自然科学基金资助项目(31000387)

Dynamic phase-contrast microscopy of living cells by digital holography

Liu Shuo, Pan Feng, Xiao Wen   

  1. School of Instrument Science and Opto-electronics Engineering, Beijing University of Aeronautics and Astronautics, Beijing 100191, China
  • Received:2011-09-20 Online:2012-09-30 Published:2012-09-27

摘要: 针对生物活体细胞的观测要求,研究了一种基于数字全息的动态相衬显微实验方法.利用光纤器件和分立光学元件搭建了一套预放大离轴数字全息实验系统,采用无穷远校正显微物镜提高了成像分辨率,同时通过曲率匹配透镜和构造数字相位掩模对相位畸变进行了校正.对小鼠活体骨细胞样品进行了连续动态观察,得到了反映细胞运动和形态变化的相衬显微图像,证明了此方法的可行性.

Abstract: A digital holographic approach for dynamic phase-contrast microscopy of living cells was proposed. Fiber optic devices and individual optical components were adopted to set up a pre-magnified off-axis digital holographic imaging system. An infinity-corrected microscope objective was utilized to improve lateral resolution. Phase aberrations were compensated by using curvature matching lens and digital phase mask. The proposed method was used to investigate living samples of mouse osteocytes, whose movements and variations were indicated by phase-contrast images. The experimental results prove the feasibility of the proposed method.

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