北京航空航天大学学报 ›› 2009, Vol. 35 ›› Issue (8): 942-945.

• 论文 • 上一篇    下一篇

定量胶体金试剂条浓度检测方法

王 帅, 郑德智, 樊尚春   

  1. 北京航空航天大学 仪器科学与光电工程学院, 北京 100191
  • 收稿日期:2008-07-03 出版日期:2009-08-31 发布日期:2010-09-16
  • 作者简介:王 帅(1984-) ,女,吉林磐石人,博士生, shuai841007@yahoo.com.cn.
  • 基金资助:

    航空科学基金资助项目(2007ZD51050)

Detecting method of quantitative colloidal gold test strip concentration

Wang Shuai, Zheng Dezhi, Fan Shangchun   

  1. School of Instrument Science and Opto-electronics Engineering, Beijing University of Aeronautics and Astronautics, Beijing 100191, China
  • Received:2008-07-03 Online:2009-08-31 Published:2010-09-16

摘要: 胶体金免疫层析浓度检测方法主要利用比色原理,当被检测液体中抗原与试剂条上抗体反应后,试剂条上检测区域颜色发生变化,与标准比色卡进行比较,即可定性分析浓度.目前胶体金试剂条大多为半定量测量,使其应用范围受到限制.采用一种基于DSP(Digital Signal Processor)的嵌入式图像处理系统,利用CMOS(Complementary Metal Oxide Semiconductor)图像传感器获取定量胶体金试剂条图像信息,根据图像特点进行Bayer变换、白平衡处理,提取出图像特征量条纹强度,建立图像特征条纹强度与样本浓度值的函数关系,实现被检测液体浓度的精确测量.与其它方法相比,具有精度高、检测速度快等优点.实验结果表明:基于DSP硬件平台的定量胶体金试剂条浓度检测精度可达到±5%,重复性误差达到±1%,具有一定的应用价值.

Abstract: Colloidal gold immunochromatography assay concentration detecting method uses the colorimetric principle, which compares with the color standard card by using color varietal detection area, after a reaction between antigen of the measured liquid and antibody of the reagent strip. It-s a qualitative concentration analysis. At present, colloidal gold test strip is mostly semi-quantitative, which tends to limit its use. It adopted an embedded image processing system which based on the digital signal processor (DSP). The image information of the quantitative colloidal gold test strip was sampled by the image sensor complementary metal oxide semiconductor (CMOS), dealt by Bayer transformation, white balance processing, image feature information of fringe intensity extraction, and then established the functional relation between image feature information of fringe intensity and sample concentration, so it can realize accurate measurement. Compared with other methods, it has advantages of high precision, fast inspection speed and so on. The results show that the method of quantitative colloidal gold test strip concentration detection has upper applied value,which based on the DSP hardware platform, it precision can be up to ±5%,repeatability error reaches ±1%.

中图分类号: 


版权所有 © 《北京航空航天大学学报》编辑部
通讯地址:北京市海淀区学院路37号 北京航空航天大学学报编辑部 邮编:100191 E-mail:jbuaa@buaa.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发