Abstract: An unique world coordiantes based global calibration method is proposed, in which double theodolites system coordinare frame is taken as the general measurment coordiante frame and the system is used to directly sample calibrations points for each vision sensor in the multi-vision inspections system. Thus the whole sensors in the inpection system can finish global calibration by one time, and no medi-coordinate-frame is needed. Therewith, transformation times of coordinate frame decreases and calibration course is facilitized. Measurement accuracy analysis of the image coordinates and object coordinates is performed on the double theodolites system, respectively, and the range of sampling calibration points for gaining high measurement accuracy of theodolite in practice is provided. Finally, global calibration experiment on a real multi-strutured light stripe vision inspection system is conducted with root mean square(RMS) error of not more than 0.24mm.