Abstract: Drawing on the resource-based view and organization learning theory, we empirically examine the relationship among entrepreneurial culture, ambidextrous innovation, marketing capability and performance of the high-tech firm. The results indicate that entrepreneurial culture is positively significantly associated with exploitative innovation, explorative innovation and marketing capability. The findings also suggest that exploitative innovation and marketing capability have a positive effect on firm performance, while explorative innovation is indirectly associated with firm performance through marketing capability. Furthermore, this research finds that environmental dynamism positively moderates the relationship between explorative innovation and firm performance, negatively moderates the relationship between exploitative innovation and firm performance, but does not have significant moderating effect on the relationship between marketing capability and firm performance. This study provides a path reference for improving ambidextrous innovation, marketing capability and firm performance of the high-tech enterprises.