Abstract: Entrepreneurship, as a new innovation element, is an accelerator and a driving force that can not be ignored in the technological innovation of Chinese enterprises. This paper carried out the single case study to investigate the role and mechanism of entrepreneurship in promoting the formation of dominant position of enterprises in innovation. The results showed that entrepreneurship accelerates the acquisition of key innovation elements such as innovative technology, innovative talents and innovative funds by building a management system conducive to innovation and creating an innovation culture of enterprises, thus helping enterprises achieve breakthroughs in technological innovation, serve the national strategy, and finally takes the dominant position in innovation in a certain industry. The study also found that the key connotations of entrepreneurship in the new era are national spirit and enterprising spirit. The findings have certain reference value for the government to cultivate entrepreneurship and select entrepreneurs, and provide a practical path for entrepreneurs to strengthen the dominant position of enterprises in innovation.