Abstract: Beijing, Shanghai, and Shenzhen, as the highland of science and technology innovation in China, have important strategic significance for innovation and development and catching up. Technology transfer is an important means of innovation and has an important impact on the improvement of innovation capabilities and economic development. Based on the panel data of Beijing, Shanghai, and Shenzhen from 2010 to 2020, this paper establishes a DEA model to measure the technology transfer efficiency of the three cities and conducts sensitivity analysis and projection value analysis at the same time to analyze the similarities and differences of the three cities. In addition to the problem of insufficient export volume of high-tech products, Beijing still has the problem of low output efficiency of R&D personnel, indicating that the development method with large-scale capital investment and personnel investment is no longer suitable for the current development stage of the three cities. In response to the above conclusions, we put forward policy recommendations such as rational planning and investment, increasing quality and efficiency, conforming to the dual-cycle development pattern, pioneering breakthroughs, focusing on key elements, and characteristic management.