Abstract: The conflict of civil jurisdiction between the mainland and Macao is interregion al caused by different legal systems under "one country, two systems", which c an be harmonized by means of unilateral approach, direct negotiation and interr egional agreements. In the process of harmonization, the principle of autonomy o f the will, protecting the legality interests of the parties, adjudicating a cas e only once, the most significant contact and consistence of exclusive jurisdict ion should be followed.