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可吸收胶原膜的体内免疫反应评价

张林 孙磊 徐梦浛 牛旭锋

张林, 孙磊, 徐梦浛, 等 . 可吸收胶原膜的体内免疫反应评价[J]. 北京航空航天大学学报, 2018, 44(4): 879-886. doi: 10.13700/j.bh.1001-5965.2017.0230
引用本文: 张林, 孙磊, 徐梦浛, 等 . 可吸收胶原膜的体内免疫反应评价[J]. 北京航空航天大学学报, 2018, 44(4): 879-886. doi: 10.13700/j.bh.1001-5965.2017.0230
ZHANG Lin, SUN Lei, XU Menghan, et al. Immunological response evaluation of absorbable collagen membrane in vivo[J]. Journal of Beijing University of Aeronautics and Astronautics, 2018, 44(4): 879-886. doi: 10.13700/j.bh.1001-5965.2017.0230(in Chinese)
Citation: ZHANG Lin, SUN Lei, XU Menghan, et al. Immunological response evaluation of absorbable collagen membrane in vivo[J]. Journal of Beijing University of Aeronautics and Astronautics, 2018, 44(4): 879-886. doi: 10.13700/j.bh.1001-5965.2017.0230(in Chinese)

可吸收胶原膜的体内免疫反应评价

doi: 10.13700/j.bh.1001-5965.2017.0230
基金项目: 

国家自然科学基金 31470915

中央高校基本科研业务费专项资金 YWF-17-BJ-Y-45

详细信息
    作者简介:

    张林  女, 硕士研究生。主要研究方向:生物医用材料

    牛旭锋  男, 博士, 副教授, 博士生导师。主要研究方向:生物医用材料

    通讯作者:

    牛旭锋, E-mail: nxf@buaa.edu.cn

  • 中图分类号: R318.06

Immunological response evaluation of absorbable collagen membrane in vivo

Funds: 

National Natural Science Foundation of China 31470915

the Fundamental Research Funds for the Central Universities YWF-17-BJ-Y-45

More Information
  • 摘要:

    免疫应答反应一直是限制可植入生物材料应用的关键因素之一。实验评估了2种用于骨修复的胶原膜在体内的免疫反应,希望为其临床试验提供依据。在将2种膜皮下植入到BALB/c小鼠后的第14天,与阴性对照(NC,未植入材料)相比,脾和淋巴结没有明显肿大,淋巴结细胞数几乎没差异,而脾细胞数大约是NC的2倍。流式细胞术分析显示植入胶原膜1导致脾中T细胞比例减少了约13%,但是没有影响T细胞亚群,而植入胶原膜2对小鼠的脾细胞组成没有明显影响;2种胶原膜都激活了一定的B细胞,激活率大约为NC小鼠的2倍。淋巴细胞体外增殖实验显示与NC组无显著差异。酶联免疫吸附测试表明胶原膜1导致了第14天血清中的抗体浓度升高至NC小鼠的2倍。局部H&E染色显示2种材料都引起了轻微的细胞浸润。这2种胶原膜引起的免疫反应很微弱,可以被应用于临床试验。

     

  • 图 1  脾和淋巴结形态

    Figure 1.  Images of spleen and lymph nodes

    图 2  脾和淋巴结的免疫细胞计数结果(***p < 0.005)

    Figure 2.  Immune cell population of spleen and lymph nodes (***p < 0.005)

    图 3  流式细胞术分析脾淋巴细胞表型

    Figure 3.  Splenic cells' phenotype analysis by flow cytometry

    图 4  CD4+T细胞、CD8+T细胞和CD19+细胞的Ki67表达情况

    Figure 4.  Ki67 expression of CD4+T lymphocytes, CD8+T lymphocytes and CD19+ lymphocytes

    图 5  脾和淋巴结细胞体外增殖情况(**p < 0.01)

    Figure 5.  Proliferation in vitro of splenic cells and lymph nodes cells (**p < 0.01)

    图 6  血清中IgG浓度随时间的变化

    Figure 6.  Variation of concentration of IgG in serum with time

    图 7  植入部位H&E染色

    Figure 7.  H&E staining of implantation sites

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出版历程
  • 收稿日期:  2017-04-13
  • 录用日期:  2017-05-19
  • 刊出日期:  2018-04-20

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