| Citation: | ZHANG Lin, SUN Lei, XU Menghan, et al. Immunological response evaluation of absorbable collagen membrane in vivo[J]. Journal of Beijing University of Aeronautics and Astronautics, 2018, 44(4): 879-886. doi: 10.13700/j.bh.1001-5965.2017.0230(in Chinese)
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Immune response is one of the critical factors to limit implantable biomaterials' application. In this study, two kinds of collagen membranes for bone regeneration were evaluated in terms of immunological response in vivo, which is hoped to lay foundations for clinical trials. At day 14 following subcutaneous implantation of the two membranes in BALB/c mice, compared with negative control (NC, no implant), spleen and lymph nodes showed no obvious swelling, and normal lymph nodes'cell population as well as one-fold more splenic cell population was observed. Flow cytometry analysis demonstrated that in mice with collagen membrane 1, splenic T lymphocytes percentage decreased by about 13%, but no significant change was discovered in T lymphocyte subsets; collagen membrane 2 did not trigger apparent splenic cells composition alteration; the two membranes activated one-fold more B lymphocytes than NC. Enzyme linked immunosorbent assay indicated that one-fold higher IgG concentration than NC was detected in mice with collagen membrane 1 in day 14. Lymphocytes proliferation assay in vitro did not show significant difference from NC. H&E staining of local tissues exhibited slight cell infiltration around the two membranes. The two collagen membranes have engendered mild immune response and can be applied to clinical trials.
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