Series microscopy images fusion based on area wavelet transform
-
摘要: 利用图像处理技术实现序列显微图像的融合显示是解决显微成像焦深有限的有效方法.针对空域融合算法在处理离焦步长较大和透明显微图像时存在的块效应和重影现象,基于图像像素间高度的相关性和小波变换多分辨率分析的特点,提出了一种基于区域小波变换的序列显微图像融合算法:在小波变换域内进行序列显微图像融合时,不同频段选择不同的融合算子;在确定融合的小波系数时,不仅考虑到相应位置的小波系数,还考虑了与它相邻的小波系数,使得融合后图像克服了空域算法存在的块效应和重影现象.同时,研究了不同的小波滤波器对图像融合质量的影响.实验证明,该方法具有很好的融合效果.Abstract: Digital image fusion technique based on multi-focus series microscopy images is an effective method to extend the depth of focus. In order to avoid the blocking effect of fused image by spatial domain fusion algorithm when large focusing step is chosen or the image samples are transparent, a series microscopy images fusion algorithm based on area wavelet transform is proposed. Considering the fact that neighboring pixels are related and wavelet transform has the multi-resolution merit, fusion strategies are as follow: fusion operators are different corresponding to different frequency region, and fusion process is based on region coefficients of wavelet transform. Meanwhile, the influences of different wavelet bases on performance of fusion are investigated. Experimental results show that the blur is improved in obtained fused image and the blocking effect is eliminated. The fusion performance of this method is satisfactory.
-
Key words:
- image fusion /
- wavelet transform /
- microscopy image /
- multi-focus
-
[1] Valdecasas A G, Marshall D. Extended depth-of-focus algorithms in brightfield microscopy[J]. Microscopy and Analysis, 2002, 29(9):13~15 [2] Hausler G. A method to increase the depth of focus by two step image processing[J]. Opt Commun, 1972, 38(6):38~42 [3] Sugimoto S A, Ichioka Y. Digital composition of images with increased depth of focus considering depth information[J]. Appl Opt, 1986, 24:2076~2084 [4] Subbarao M, Tao Choi. Accurate recovery of three-dimensional shape from image focus[J]. Pattern Analysis and Machine Intelligence, 1995, 17:266~274 [5] Asif M, Tae-Sun Choi. Learning shape from focus using multilayer neural networks . SPIE , 1999,3811:366~375 [6] Valdecasas A G, Marshall D. On the extended depth of focus algorithms for bright field microscopy[J]. Micron, 2001,32:559~569 [7] Nayar S K, Nakagawa Yasuo. Shape from focus[J]. IEEE Transactions on Pattern Analysis and Machine Intelligence, 1994, 16(8):824~830 [8] 姜志国,史文华,韩冬兵,等.基于聚焦合成的显微三维成像系统[J].CT理论与应用研究,2004,13(4):9~15 Jiang Zhiguo, Shi Wenhua, Han Dongbing, \%et al\%. Three-dimensional microscopy image system base on depth from focus[J]. Computerized Tomography Theory and Applications, 2004,13(4):9~15(in Chinese) [9] 杨福生.小波变换的工程分析与应用[M].北京:科学出版社,1999.134~139 Yang Fusheng. Wavelet transform engineering analysis and application[M]. Beijing:Science Press, 1999. 134~139(in Chinese) -
点击查看大图
计量
- 文章访问数: 2944
- HTML全文浏览量: 151
- PDF下载量: 1199
- 被引次数: 0
下载:
