Improved modeling strategy and result evaluation of steroid-induced femoral head necrosis in rabbits
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摘要:
股骨头坏死动物模型的建立对临床上股骨头坏死的治疗具有重要意义。对目前最常见的兔激素性股骨头坏死的造模方法进行改进，并对改进后的造模结果进行评估，希望能够在降低造模动物死亡率的同时成功诱导股骨头坏死。选择周龄介于16~20周，体重介于2.8~3.2 kg的雄性新西兰大白兔90只，随机分为空白对照组、常规造模组和造模改进组3组。其中，空白对照组仅注射生理盐水；常规造模组在特定时间点注射脂多糖和甲基泼尼松龙；造模改进组在常规造模组的基础上辅以青霉素、雷尼替丁、地克珠利、肠道润滑剂和乳酶生干预。结果表明：造模改进组动物的死亡率（13.3%）远低于常规造模组（70%）。造模完成后的微型计算机断层扫描技术（Micro-CT）分析和组织学染色结果显示：造模改进组股骨头区域出现了早期股骨头坏死的特征，其骨体积、骨体积与组织体积比、骨密度、骨小梁厚度均显著低于空白对照组，而空骨陷窝个数相较于空白对照组则出现了显著提升，证明改进后的造模方法在降低死亡率的同时成功诱导了兔股骨头坏死。
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关键词:
- 股骨头坏死 /
- 激素 /
- 动物模型 /
- 微型计算机断层扫描技术 /
- 组织学染色
Abstract:The establishment of animal model of femoral head necrosis is of great significance for the clinical treatment of this disease. To successfully induce femoral head necrosis while reducing the mortality of modeling animals, this study improves the commonly used modeling method of steroid-induced femoral head necrosis in rabbits. Ninety male New Zealand white rabbits (2.8~3.2 kg) are randomly divided into control group, conventional model group and improved model group. The control group is only injected with normal saline; the conventional model group is injected with lipopolysaccharide and methylprednisolone; the improved model group is supplemented with penicillin, ranitidine, diclazuril, intestinal lubricant and lactasin based on conventional model. The results show that the death rate of the improved model group (13.3%) is much lower than that of the conventional model group (70%). The Micro-CT and histological results exhibit that the bone volume, bone volume/tissue volume ratio, bone mineral density and trabecular thickness in improved model group are significantly lower than those in control group, while the number of empty bone lacunae is significantly higher than that in control group. These results demonstrate the successful establishment of steroid-induced femoral head necrosis in rabbits.
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Key words:
- femoral head necrosis /
- steroid /
- animal model /
- Micro-CT /
- histological staining
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图 1 激素性股骨头坏死动物模型造模过程中死亡因素
Figure 1. Death factors in animal model of steroid-induced femoral head necrosis
图 3 股骨头部位Micro-CT断层扫描及三维重建图像
Figure 3. Micro-CT tomography and 3D reconstruction images of femoral head
图 4 股骨头部位Micro-CT数据定量分析结果
Figure 4. Quantitative analysis of Micro-CT data in femoral head region
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