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兔激素性股骨头坏死的造模改进及结果评价

杨丰合 冯桂宇 李晋玉 牛旭锋

杨丰合,冯桂宇,李晋玉,等. 兔激素性股骨头坏死的造模改进及结果评价[J]. 北京航空航天大学学报,2023,49(7):1755-1760 doi: 10.13700/j.bh.1001-5965.2021.0558
引用本文: 杨丰合,冯桂宇,李晋玉,等. 兔激素性股骨头坏死的造模改进及结果评价[J]. 北京航空航天大学学报,2023,49(7):1755-1760 doi: 10.13700/j.bh.1001-5965.2021.0558
YANG F H,FENG G Y,LI J Y,et al. Improved modeling strategy and result evaluation of steroid-induced femoral head necrosis in rabbits[J]. Journal of Beijing University of Aeronautics and Astronautics,2023,49(7):1755-1760 (in Chinese) doi: 10.13700/j.bh.1001-5965.2021.0558
Citation: YANG F H,FENG G Y,LI J Y,et al. Improved modeling strategy and result evaluation of steroid-induced femoral head necrosis in rabbits[J]. Journal of Beijing University of Aeronautics and Astronautics,2023,49(7):1755-1760 (in Chinese) doi: 10.13700/j.bh.1001-5965.2021.0558

兔激素性股骨头坏死的造模改进及结果评价

doi: 10.13700/j.bh.1001-5965.2021.0558
基金项目: 国家自然科学基金(11872097,82074463);国家重点研发计划(2020YFC0122204);111计划(B13003)
详细信息
    通讯作者:

    E-mail:nxf@buaa.edu.cn

  • 中图分类号: R681.8;R318

Improved modeling strategy and result evaluation of steroid-induced femoral head necrosis in rabbits

Funds: National Natural Science Foundation of China (11872097,82074463); National Key Research and Development Program of China (2020YFC0122204); 111 project (B13003)
More Information
  • 摘要:

    股骨头坏死动物模型的建立对临床上股骨头坏死的治疗具有重要意义。对目前最常见的兔激素性股骨头坏死的造模方法进行改进,并对改进后的造模结果进行评估,希望能够在降低造模动物死亡率的同时成功诱导股骨头坏死。选择周龄介于16~20周,体重介于2.8~3.2 kg的雄性新西兰大白兔90只,随机分为空白对照组、常规造模组和造模改进组3组。其中,空白对照组仅注射生理盐水;常规造模组在特定时间点注射脂多糖和甲基泼尼松龙;造模改进组在常规造模组的基础上辅以青霉素、雷尼替丁、地克珠利、肠道润滑剂和乳酶生干预。结果表明:造模改进组动物的死亡率(13.3%)远低于常规造模组(70%)。造模完成后的微型计算机断层扫描技术(Micro-CT)分析和组织学染色结果显示:造模改进组股骨头区域出现了早期股骨头坏死的特征,其骨体积、骨体积与组织体积比、骨密度、骨小梁厚度均显著低于空白对照组,而空骨陷窝个数相较于空白对照组则出现了显著提升,证明改进后的造模方法在降低死亡率的同时成功诱导了兔股骨头坏死。

     

  • 图 1  激素性股骨头坏死动物模型造模过程中死亡因素

    Figure 1.  Death factors in animal model of steroid-induced femoral head necrosis

    图 2  各组造模死亡率随时间变化曲线

    Figure 2.  Curves of death rate with time in different groups

    图 3  股骨头部位Micro-CT断层扫描及三维重建图像

    Figure 3.  Micro-CT tomography and 3D reconstruction images of femoral head

    图 4  股骨头部位Micro-CT数据定量分析结果

    Figure 4.  Quantitative analysis of Micro-CT data in femoral head region

    图 5  股骨头部位H&E染色图像及空骨陷窝数目统计

    Figure 5.  H&E staining images and the number of empty bone lacunae in femoral head region

  • [1] WANG C K, HO M L, WANG G J, et al. Controlled-release of rhBMP-2 carriers in the regeneration of osteonecrotic bone[J]. Biomaterials, 2009, 30(25): 4178-4186. doi: 10.1016/j.biomaterials.2009.04.029
    [2] MARUYAMA M, NABESHIMA A, PAN C C, et al. The effects of a functionally-graded scaffold and bone marrow-derived mononuclear cells on steroid-induced femoral head osteonecrosis[J]. Biomaterials, 2018, 187: 39-46. doi: 10.1016/j.biomaterials.2018.09.030
    [3] ZHANG H X, ZHANG X P, XIAO G Y, et al. In vitro and in vivo evaluation of calcium phosphate composite scaffolds containing BMP-VEGF loaded PLGA microspheres for the treatment of avascular necrosis of the femoral head[J]. Materials Science and Engineering:C, 2016, 60: 298-307. doi: 10.1016/j.msec.2015.11.055
    [4] WAEWSAWANGWONG W, RUCHIWIT P, HUDDLESTON J I, et al. Hip arthroplasty for treatment of advanced osteonecrosis: Comprehensive review of implant options, outcomes and complications[J]. Orthopedic Research and Reviews, 2016, 8: 13-29. doi: 10.2147/ORR.S35547
    [5] STEVENS K, TAO C, LEE S U, et al. Subchondral fractures in osteonecrosis of the femoral head: Comparison of radiography, CT, and MR imaging[J]. American Journal of Roentgenology, 2003, 180(2): 363-368. doi: 10.2214/ajr.180.2.1800363
    [6] QIN L, ZHANG G, SHENG H, et al. Multiple bioimaging modalities in evaluation of an experimental osteonecrosis induced by a combination of lipopolysaccharide and methylprednisolone[J]. Bone, 2006, 39(4): 863-871. doi: 10.1016/j.bone.2006.04.018
    [7] 孙磊, 袁源, 牛睿, 等. 辐照和EO灭菌对SIS材料免疫原性的对比研究[J]. 北京航空航天大学学报, 2020, 46(12): 2245-2252. doi: 10.13700/j.bh.1001-5965.2019.0621

    SUN L, YUAN Y, NIU R, et al. Comparative study on immunoreactions of small intestinal submucosa by irradiation and ethylene oxide sterilization treatments[J]. Journal of Beijing University of Aeronautics and Astronautics, 2020, 46(12): 2245-2252(in Chinese). doi: 10.13700/j.bh.1001-5965.2019.0621
    [8] 张林, 孙磊, 徐梦浛, 等. 可吸收胶原膜的体内免疫反应评价[J]. 北京航空航天大学学报, 2018, 44(4): 879-886. doi: 10.13700/j.bh.1001-5965.2017.0230

    ZHANG L, SUN L, XU M H, et al. Immunological response evaluation of absorbable collagen membrane in vivo[J]. Journal of Beijing University of Aeronautics and Astronautics, 2018, 44(4): 879-886(in Chinese). doi: 10.13700/j.bh.1001-5965.2017.0230
    [9] XU J Z, GONG H P, LU S T, et al. Animal models of steroid-induced osteonecrosis of the femoral head-a comprehensive research review up to 2018[J]. International Orthopaedics, 2018, 42(7): 1729-1737. doi: 10.1007/s00264-018-3956-1
    [10] 陈冠儒, 王萧枫, 王利明, 等. 兔激素性股骨头坏死造模中的致死因素与干预分析[J]. 浙江中西医结合杂志, 2012, 22(5): 342-344. doi: 10.3969/j.issn.1005-4561.2012.05.004

    CHEN G R, WANG X F, WANG L M, et al. Analysis on fetal factors and intervention of steriod-induced femoral head necrosis in rabbits[J]. Zhejiang Journal of Integrated Traditional Chinese and Western Medicine, 2012, 22(5): 342-344(in Chinese). doi: 10.3969/j.issn.1005-4561.2012.05.004
    [11] 王程, 苟文隆, 徐小龙, 等. 人股骨头坏死标本不同区域骨小梁的显微结构特征及病理学表现[J]. 解放军医学院学报, 2014, 35(5): 463-465. doi: 10.3969/j.issn.2095-5227.2014.05.018

    WANG C, GOU W L, XU X L, et al. Microstructure features and pathology of trabecula in different regions of femoral head necrosis[J]. Academic Journal of Chinese PLA Medical School, 2014, 35(5): 463-465(in Chinese). doi: 10.3969/j.issn.2095-5227.2014.05.018
    [12] HAMBLI R. Micro-CT finite element model and experimental validation of trabecular bone damage and fracture[J]. Bone, 2013, 56(2): 363-374. doi: 10.1016/j.bone.2013.06.028
    [13] ZHANG G, SHENG H, HE Y X, et al. Continuous occurrence of both insufficient neovascularization and elevated vascular permeability in rabbit proximal femur during inadequate repair of steroid-associated osteonecrotic lesions[J]. Arthritis and Rheumatism, 2009, 60(10): 2966-2977. doi: 10.1002/art.24847
    [14] LAI Y X, LI Y, CAO H J, et al. Osteogenic magnesium incorporated into PLGA/TCP porous scaffold by 3D printing for repairing challenging bone defect[J]. Biomaterials, 2019, 197: 207-219. doi: 10.1016/j.biomaterials.2019.01.013
    [15] HA Y C, KIM H J, KIM S Y, et al. Effects of age and body mass index on the results of transtrochanteric rotational osteotomy for femoral head osteonecrosis: Surgical technique[J]. The Journal of Bone and Joint Surgery American Volume, 2011, 93(S1): 75-84.
    [16] JIN S Y, MENG C Q, HE Y, et al. Curcumin prevents osteocyte apoptosis by inhibiting M1-type macrophage polarization in mice model of glucocorticoid-associated osteonecrosis of the femoral head[J]. Journal of Orthopaedic Research, 2020, 38(9): 2020-2030. doi: 10.1002/jor.24619
    [17] WU X, FENG X, HE Y, et al. IL-4 administration exerts preventive effects via suppression of underlying inflammation and TNF-alpha-induced apoptosis in steroid-induced osteonecrosis[J]. Osteoporosis International, 2016, 27(5): 1827-1837. doi: 10.1007/s00198-015-3474-6
    [18] WANG C Q, XU H X, LIU C, et al. CaO2/gelatin oxygen slow-releasing microspheres facilitate tissue engineering efficiency for the osteonecrosis of femoral head by enhancing the angiogenesis and survival of grafted bone marrow mesenchymal stem cells[J]. Biomaterials Science, 2021, 9(8): 3005-3018. doi: 10.1039/D0BM02071K
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出版历程
  • 收稿日期:  2021-09-17
  • 录用日期:  2021-10-22
  • 网络出版日期:  2021-12-22
  • 整期出版日期:  2023-07-31

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